ABSTRACT
The Indonesian leaffish Pristolepis grootii (Bleeker, 1852) is an indigenous fish species. The purpose of this study was to analyze the characteristics of the β-subunit of follicle-stimulating hormone (FSH-β) and the β-subunit of luteinizing hormone (LH-β) of P. grotii. Complementary DNA (cDNA) was synthesized from mRNA that was extracted from P. grootii pituitary glands. Twelve fish were reared in two aquaria and injected with luteinizing hormone-releasing hormone analog (LHRHa) for the analysis of FSH-β and LH-β gene expression. The results showed that P. grootii FSH-β was 227 bp in length, consists of 93 amino acid residues, while LH-β was 300 bp in length and consists of 100 amino acid residues. P. grootii FSH revealed the highest similarity (91%) with climbing perch Anabas testudineus, while LH-β with Betta splendens (90%). Two putative N-glycosylation sites in FSH-β and one site in LH-β were conserved in P. grootii. The expression of FSH-β and LH-β in fish injected with LHRHa was higher than that of LHRHa not injected fish. The ovary of P. grootii had various sizes of oocytes. The injection of LHRHa affected the development of oocytes; there was a trend for higher doses LHRHa to develop oocytes faster. Thus, it is most likely that the isolated genes are FSH-β and LH-β of P. grootii.
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