Ovarian development of Notopterus notopterus (Pallas, 1769) injected with human chorionic gonadotropin

 

Abstract. 

The bronze featherback, Notopterus notopterus (Pallas, 1769), is a wild species that has not been domesticated. Wild species reared in aquaculture tanks tend to experience disturbances in their reproductive systems. The purpose of this study was to determine changes in the development of N. notopterus ovaries after being injected with human chorionic gonadotropin (hCG). This study was a laboratory experiment. The experiment used a completely randomized design with four treatments and three replications of hCG dosage (0, 150, 200, and 250 IU kg^-1 of fish). Administration of hCG was through intramuscular injection. Each fish was injected only once. On the 7th and 14th days after injection, blood was collected from N. notopterus, and then the fish were dissected for liver and ovary collection. The results showed that the administration of hCG stimulated the development of N. notopterus ovaries. The stage of ovarian maturity in N. notopterus increased. This is the first study to induce ovarian maturation in N. notopterus using hCG. The results of this study provide valuable information for future studies on the breeding programs of N. notopterus.

Key Words: fish reproduction, Notopteridae, oocyte diameter, ovary maturation, ovisomatic index.

 

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How to cite this article: 

Muslim M., Syaifudin M., Taqwa F. H., Nabilah F., 2025 Ovarian development of Notopterus notopterus (Pallas, 1769) injected with human chorionic gonadotropin. AACL Bioflux 18(3):1361-1369.

Effects of Purwoceng Plant (Pimpinella alpine) Extract on Masculinization of Guppy (Poecilia reticulata)

 ABSTRACT

In the context of guppies (Poecilia reticulata), male fish are considered more attractive and valuable. The majority of mono-sex production in male fish employs the use of 17α-methyltestosterone, an expensive hormone with limited supply. It is therefore necessary to explore the potential of natural ingredients as an alternative replacement, such as purwoceng (Pimpinella alpina). The objective of this study was to determine the optimal immersion duration for the masculinization of P. reticulata. This study employed a completely randomized design with four treatments of immersion duration (0, 8, 10, and 12 hours), with three replications allocated to each treatment. Following immersion, the guppy larvae were reared for a period of 45 days and fed with Daphnia, sp and Artemia cysts. The sex of each fish was determined via surgical procedure at the conclusion of the larval rearing period. The findings revealed that the duration of larval immersion in P. alpina extract had a notable impact (P < 0.05) on the proportion of male fish. In this study, the 10-hour immersion treatment yielded the highest number of male P. reticulata, at 83.33%. The results of this study are highly valuable for the application of sex reversal techniques using phytosterol as a replacement for synthetic hormones.

Keywords: mono sex culture, phytosterol, purwoceng, sex reversal, stigmasterol

 

Journal of Applied Biology & Biotechnology

 Acceptance - (JABB-2024-07-516)