ABSTRACT
In the context of guppies (Poecilia reticulata), male fish are considered
more attractive and valuable. The majority of mono-sex production in male fish
employs the use of 17α-methyltestosterone, an expensive hormone with limited
supply. It is therefore necessary to explore the potential of natural
ingredients as an alternative replacement, such as purwoceng (Pimpinella alpina). The objective of
this study was to determine the optimal immersion duration for the
masculinization of P. reticulata. This study employed a completely randomized
design with four treatments of immersion duration (0, 8, 10, and 12 hours),
with three replications allocated to each treatment. Following immersion, the
guppy larvae were reared for a period of 45 days and fed with Daphnia, sp and Artemia cysts. The sex of each fish was determined via surgical
procedure at the conclusion of the larval rearing period. The findings revealed
that the duration of larval immersion in P. alpina extract had a notable impact (P
< 0.05) on the proportion of male fish. In this study, the 10-hour immersion
treatment yielded the highest number of male P. reticulata, at 83.33%. The results of this
study are highly valuable for the application of sex reversal techniques using
phytosterol as a replacement for synthetic hormones.
Journal
of Applied Biology & Biotechnology
Acceptance - (JABB-2024-07-516)
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